v3 · Ready for sequencingCurrent
Saved by Maya · Sep 16
A complete data trail from day one.
Plasmole records who designed each construct, what changed in each version, and which experiments and results used it.
Derived from pET-28a and scFv by Gibson assembly
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- Sequence verification
Entry · used v3
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v2 · Annotated construct
Saved by Maya · Sep 2
- scFv-F / scFv-R
Primers · used v2
- Colony PCR
Entry · used v2
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v1 · Gibson assembly
Construct created · Aug 19
From starting sequence to final construct, in one place.
Work by hand, with AI, or both. The trail is the same either way.
Start from protein · anti-HER2 scFvPriya · by hand
Input245 aa
Codon tableE. coli
Back-translation check245 of 245 aa match
GC content51 %
Added to the trailv1 · codon-optimized, saved by Priya
Synthesis check · scFv-Ecolibefore ordering
Single-base runs, 8+none
Direct repeats, 20+ bp1 · 412–437 · flagged
Inverted repeats, 20+ bpnone
GC per 50 bp window34–68 %
Added to the trailv2 · repeat fixed by Priya
Gibson assembly · into pET-28aPriya · with AI
Overlaps2 · 25 bp each
ProductpET-28a-scFv · 5,954 bp
Simulated gel · NcoI + XhoI5,219 + 735 bp
Design checkpassed
Added to the trailv3 · AI plan approved by Priya
Primers · scFv-F / scFv-RPlasmole AI · Primer3
scFv-F45 nt · Tm 62 °C
scFv-R44 nt · Tm 61 °C
Hairpinsnone flagged
Primer dimersnone flagged
Added to the trailpair linked to v3
Working with your CRO · pET-28a-scFvSep 2 · shared link
Shared link · constructrevocable
Sanger batch from the CRO12 clones aligned
Match the design11 of 12
clone_7.ab11 mismatch · flagged
Added to the trailalignment linked to v3
Translate, align and codon-optimize for your host.
Catch long single-base runs, repeats and GC extremes before you order synthesis.
Gibson, Golden Gate or restriction cloning, checked on a simulated gel.
Tm, hairpins and primer dimers, worked out before the oligos ship.
Share the construct with your CRO, then align their Sanger reads to the design.
01 Provenance
A record you can stand behind.
- 01
Every version says who and when.
Changes the AI made are labelled as the AI's, not as a person's.
- 02
A saved notebook version can't be rewritten.
Not by a user, an admin or us.
- 03
Every record is linked both ways.
Every entry points to the construct, protocol and gel behind it, and they point back.
02 Diligence
Ready when someone asks how it was made.
When you file a patent or a regulatory submission, the record of how each construct was made is already written down.
Your work stays yours
No model training on it, open export formats and a public subprocessor list. Security overview
What isn't done yet
A SOC 2 Type I audit is in progress, and there is no report yet.
03 Questions
How long does setup take?
Create a team workspace, invite the team and import your files. Team workspaces are on paid plans.
Can we share work with our CROs?
Yes. Send a construct, notebook entry or workflow as a link and revoke it when the work is done. When their Sanger reads come back, align them to the design.
When do SSO and a SOC 2 report arrive?
Your team can sign in with Google today. Company single sign-on (SAML and OIDC, through your own identity provider) is on our 2026–2027 roadmap. A SOC 2 Type I audit is in progress; until the report exists, we answer security questionnaires under NDA.
How is this different from enterprise lab platforms?
Enterprise platforms are built for large R&D organizations and usually start with an implementation project. Plasmole puts design tools, the notebook and the agent in one workspace a small team can start using without a services project.