Edit and annotate a plasmid

Every plasmid opens in the editor with a map and side panes. Bases are locked until you unlock them; features can be added at any time.

Open and view a plasmid

1. Open the plasmid from the Library.
2. Use the tab strip to switch between Circular and Linear views.
3. Open the Features, Enzymes, Primers, Provenance or Selection pane beside the map.

Edit the sequence

1. Click Sequence Locked in the tab strip.
2. In Unlock sequence editing?, click Unlock sequence.
3. Click in the sequence and type A, C, G, T or N. Select bases and type to replace them, or press Backspace to delete.
4. Press Ctrl/Cmd+Z to undo and Ctrl/Cmd+Shift+Z to redo.

Changes save automatically. Each save is kept in Use version history.

Add a feature from a selection

1. Drag across the map or sequence to select a region.
2. Right-click and choose Create, then Feature from Selection.
3. Fill in Name and Type, pick Forward or Reverse, and click Save Feature.

You can also run Add Feature from the command palette (Ctrl/Cmd+K).

Find features automatically

1. Click Editor on the left rail to open the tools list. Under Design, choose Auto-Annotate Features.
2. Keep Match known parts on to compare against about 1,000 common plasmid parts.
3. Keep ORFs on and set Min ORF length. Click Re-analyze to apply after changes.
4. Tick the features you want and click Add N features.

To annotate many plasmids, select them in the Library and click Auto-annotate.

Copy part of a sequence

1. Select a region or a feature and right-click.
2. Under Copy, choose DNA Sequence, Reverse Complement, FASTA or Amino Acid Translation.

Move the origin

1. Unlock the sequence.
2. On a circular map, right-click the base that should become position 1.
3. Choose Set as Sequence Origin.